alphagenome — Claude Code skill: what it does, how to install

---
name: alphagenome
description: "Looks up precomputed AlphaGenome Atlas effects for any GRCh38 single-nucleotide variant (AVI score with Phred and 18 SHAP feature attributions, plus raw and quantile scores for RNA-seq, DNase, ATAC, ChIP-TF, ChIP-histone, CAGE, PRO-cap, splicing, polyadenylation and contact-map tracks), scores variants or scans windows on demand with the AlphaGenome model for human and mouse (variant scoring, in silico mutagenesis, REF-versus-ALT track prediction), and builds Atlas website deep links. Use when the user mentions AlphaGenome, AlphaGenome Atlas, AVI or AlphaGenome Variant Impact, DeepMind variant effect prediction, or wants to prioritise or mechanistically interpret non-coding, regulatory, splicing, enhancer, promoter, or chromatin-accessibility effects of SNVs from a VCF, credible set, or region. Research use only; not a clinical tool."
license: MIT
compatibility: "Python 3.10+ with the alphagenome package (0.9.0 or later for the Atlas client; brings numpy, pandas, anndata, grpcio). Network access to gdmscience.googleapis.com:443 and a free non-commercial AlphaGenome API key in ALPHAGENOME_API_KEY (ALPHA_GENOME_API_KEY also read). Human data is GRCh38 only; mouse is mm10 (model only)."
allowed-tools: Read Write Edit Bash
metadata:
  version: "1.2"
  skill-author: K-Dense Inc.
  upstream-version: "alphagenome 0.9.0"
  last-reviewed: "2026-09-30"
  openclaw:
    primaryEnv: ALPHAGENOME_API_KEY
    envVars:
    - name: ALPHAGENOME_API_KEY
      required: true
      description: AlphaGenome API key, free for non-commercial use from deepmind.google.com/science/alphagenome. ALPHA_GENOME_API_KEY is accepted as an alternative spelling.
---

# AlphaGenome and the AlphaGenome Atlas

AlphaGenome is DeepMind's sequence-to-function model: 1 Mb of DNA in,
predictions at modality-specific resolutions for eleven assay types across
thousands of human and mouse tracks out. The **AlphaGenome Atlas** (released 2026-09-08) is that model run once over
every possible single-nucleotide change in GRCh38, about 9 billion variants,
stored with a single ranking number, the **AlphaGenome Variant Impact (AVI)**
score, its genome-wide percentile, and an 18-way attribution of what drives it.
Both are reached through one `pip install alphagenome` and one API key.

> Research and theoretical modelling only. API outputs must not be used to train
> other models, and are not for diagnostic procedures or medical decisions.
> Permissive-use downloads have separate terms; check the artifact licence.

## When to use which

| You have | Use | Why |
| --- | --- | --- |
| hg38 SNVs (a VCF, a credible set, a region up to ~1 kb) | **Atlas** via `scripts/atlas_query.py` | precomputed, higher quota, includes AVI and attributions |
| indels, mouse variants, a non-reference background, a custom scorer or window | **model** via `scripts/score_variants.py` or Python | the Atlas is SNV-only and hg38-only |
| a hypothesis to explain (which motif, which tissue, REF vs ALT tracks) | model `predict_variant` + plots, Atlas track scores, portal link | mechanism, not just rank |
| GRCh37 coordinates, rsIDs, unnormalised indels | `genomic-coordinates` first, then come back | wrong build or swapped REF gives a plausible wrong answer |
| ClinVar assertions, gene-disease validity, ACMG framing | `folklore-variant-evidence`, `database-lookup` | AlphaGenome is one evidence line, never the verdict |
| promoter/enhancer/expression predictions without a DeepMind key | `genomic-intelligence` | different provider, keyless demo tier |

## Setup

```bash
uv pip install alphagenome                     # PyPI; tested on Python 3.12 and 3.13, alphagenome 0.9.0
export ALPHAGENOME_API_KEY="..."               # https://deepmind.google.com/science/alphagenome
cd skills/alphagenome/scripts
python atlas_query.py scorers                  # proves key + network in one call
```

Shell examples quote variant strings because `>` otherwise redirects output.
Network examples below are illustrative and were checked against SDK 0.9.0
contracts, without authenticated prediction/Atlas calls during this review.

Never put the key on a command line or in a file you commit; the scripts only
read it from the environment. Authentication failures may surface as
`ValueError` or `PermissionError`,
depending on the gRPC status returned by the service.

## The coordinate contract

- A variant is **1-based** `chr:pos:ref>alt` (`chr22:36201698:A>C`). gnomAD
  (`22-36201698-A-C`), GTEx (`chr22_36201698_A_C_b38`), and Open Targets
  spellings are autodetected by the scripts. In the SDK, pass the matching
  `variant_format=genome.VariantFormat.GNOMAD` (or `GTEX`, `OPEN_TARGETS`,
  `OPEN_TARGETS_BIGQUERY`); `from_str` defaults to `chr:pos:ref>alt`.
- An interval on the command line is **1-based closed** `chr:start-end`; the
  SDK's `genome.Interval` is **0-based half-open**. The scripts convert.
- Human is **GRCh38 only**. The Atlas key is `chr:pos:alt`; REF is implied by
  the reference. Swapping REF/ALT can cause a miss; a wrong REF or build can
  misidentify a record. The scripts reject a returned variant that differs
  from the request, but cannot validate the build. Check REF against GRCh38 FASTA.
- rsIDs are not accepted by the API or the portal. Resolve them to coordinates.
- Use the `chr` prefix; `MT` becomes `chrM`.

## Atlas workflow

### 1. Rank with AVI

```bash
python atlas_query.py avi --variant "chr22:36201698:A>C" "chr9:128225994:G>A"
python atlas_query.py avi --input candidates.vcf --min-phred 20 -o avi.tsv
python atlas_query.py avi --interval chr11:5225727-5226575 --top-k 25 -o hbb_window.tsv
python atlas_query.py avi --input credible_set.tsv --with-tracks -o avi_tracks.tsv
```

Output, one row per variant:

| Column | Meaning |
| --- | --- |
| `avi_raw` | composite model output (the 18 attributions sum to it) |
| `avi_cdf_quantile` | cumulative quantile against all genome-wide SNVs, as served |
| `avi_tail_quantile`, `avi_phred`, `avi_top_percent` | `tail = 1 - cdf`, `phred = -10 log10(tail)`; Phred 20 = top 1 %, 30 = top 0.1 % |
| `top_feature`, `top_feature_value` | largest absolute SHAP attribution and its value |
| `fi_MERGED_SPLICING` ... `fi_IS_DELETION` | all 18 attributions (keys in `references/atlas.md`) |
| `top_track_*` (with `--with-tracks`) | the strongest track behind the top feature: scorer, track, biosample, ontology CURIE, gene, raw score |
| `atlas_url` | deep link to the variant on the portal |
| `error` | per-variant lookup failure or mismatched returned allele instead of a crash |

The Atlas report's advice: **rank, do not threshold**, and pick thresholds by
region or application. Pathogenic regulatory variants sit in lower AVI bins
than protein-truncating or splice-motif variants, so a single genome-wide
cut-off under-calls exactly the variants this resource was built for.

Read the attribution before the number. `MERGED_SPLICING` or `ALPHAMISSENSE`
on top means a splice or coding mechanism; `MAX_ABS_DNASE`, `MAX_ABS_CHIP_TF`,
`MAX_ABS_RNA_SEQ` mean a regulatory mechanism you can resolve by track;
`CACTUS_241_WAY` or `PHASTCONS_470_WAY` on top means conservation is carrying
the score and the molecular mechanism is not resolved.

### 2. Resolve the mechanism by track

```bash
python atlas_query.py scorers                                   # what the server serves right now
python atlas_query.py tracks --scorer RNA_SEQ --query colon     # find ontology CURIEs
python atlas_query.py scores --variant "chr22:36201698:A>C" \
    --scorers RNA_SEQ DNASE SPLICE_SITE_USAGE --ontology UBERON:0001157 -o colon.tsv
python atlas_query.py scores --interval chr11:5225727-5226575 --scorers CHIP_TF -o hbb_tf.tsv
```

One row per variant x track (x gene or splice junction where applicable),
with `raw_score` and, where served, `quantile_score`. Track-level
scorer names: `ATAC`, `DNASE`, `CHIP_TF`, `CHIP_HISTONE`, `CAGE`, `PROCAP`,
`RNA_SEQ`, `POLYADENYLATION`, `SPLICE_SITES`, `SPLICE_SITE_USAGE`,
`SPLICE_JUNCTIONS`, `CONTACT_MAPS`, plus `*_ACTIVE` variants; `scorers` is the
authority on the live list. Filter by the tissue the question is about, not
by the genome-wide maximum: 9,440 scorer-track entries mean something may be extreme
somewhere.

### 3. Send the reader to the portal

```bash
python atlas_link.py variant "chr22:36201698:A>C" --biosample "colon" --modalities RNA_SEQ,DNASE,CHIP_TF
python atlas_link.py locus chr11:5225727-5226575 --tf GATA1
python atlas_link.py gene HBB --markdown
```

No key, no network. The site shows the AVI track, per-modality heatmaps over
every biosample, REF-vs-ALT prediction tracks, and motif instances. Attach a
link to every variant you report.

### In Python

```python
import os
from alphagenome.atlas import atlas
from alphagenome.data import genome

client = atlas.create(os.environ["ALPHAGENOME_API_KEY"], timeout=30)
scores = client.query_variant(
    genome.Variant.from_str("chr22:36201698:A>C"),
    requested_scorers=["AVI_SCORE", "AVI_SCORE_FEATURE_IMPORTANCE", "RNA_SEQ"],
    ontology_terms=["UBERON:0001157"],          # optional; ignored for scorers without ontology metadata
)
avi = scores["AVI_SCORE"]                        # AnnData: X (1,1) raw; layers['quantiles'] (1,1) cdf
fi = scores["AVI_SCORE_FEATURE_IMPORTANCE"]      # AnnData: X (1,18); var['name'] = feature keys
rna = scores["RNA_SEQ"]                          # AnnData: obs = variant x gene, var = tracks, X = natural-log FC
client.query_interval(genome.Interval("chr11", 5225726, 5226575), requested_scorers=["AVI_SCORE"])
```

`query_interval` returns up to 3 SNVs per non-`N` base, in 32 bp chunks. Keep windows
to about 1 kb (3,000 variants); `atlas_query.py` refuses more unless
`--max-window` is raised. `query_variants` raises if any submitted lookup fails;
completion order is not input order;
the script makes separate variant requests so misses become `error` cells.

## Model workflow

### Score variants the Atlas does not hold

```bash
python score_variants.py --variant "chr22:36201698:A>C" -o scores.tsv                   # 12 recommended scorers, 1 Mb
python score_variants.py --input indels.vcf --scorers RNA_SEQ SPLICE_SITE_USAGE \
    --ontology UBERON:0001157 --min-abs-quantile 0.99 -o colon.tsv
python score_variants.py --organism mouse --variant "chr7:45000000:A>G" --scorers RNA_SEQ --sequence-length 500KB -o mouse.tsv
python score_variants.py --list-scorers
python score_variants.py --list-tracks --output-type RNA_SEQ --query liver -o tracks.tsv
```

Output is the official tidy table from `variant_scorers.tidy_scores`: one row
per variant x scorer x track (x gene) with `raw_score` and `quantile_score`,
sorted by |raw|. Default scorers are the 12 recommended difference scorers;
`--include-active` adds the seven `*_ACTIVE` activity scorers. At most 20
scorers per request. Mouse has no calibrated quantiles; use raw scores there.
`--min-abs-quantile` uses the supplied signed quantile magnitude (unsigned
scorers use their upper-tail quantile); it never treats zero as an extreme.

```python
from alphagenome.models import dna_client, variant_scorers
model = dna_client.create(os.environ["ALPHAGENOME_API_KEY"])
variant = genome.Variant.from_str("chr22:36201698:A>C")
interval = variant.reference_interval.resize(dna_client.SEQUENCE_LENGTH_1MB)
adatas = model.score_variant(interval, variant, variant_scorers=[variant_scorers.RECOMMENDED_VARIANT_SCORERS["RNA_SEQ"]])
df = variant_scorers.tidy_scores(adatas)         # filter df.ontology_curie afterwards; score_variant takes no ontology_terms
```

### Predict tracks and mutagenise

```python
vo = model.predict_variant(interval, variant,
                           requested_outputs=[dna_client.OutputType.RNA_SEQ, dna_client.OutputType.DNASE],
                           ontology_terms=["UBERON:0001157"])
vo.reference.rna_seq.values, vo.alternate.rna_seq.values      # (1048576, n_tracks)

window = genome.Interval("chr20", 3_753_000, 3_753_400).resize(dna_client.SEQUENCE_LENGTH_16KB)
ism = model.score_ism_variants(interval=window, ism_interval=window.resize(256),
                               variant_scorers=[variant_scorers.CenterMaskScorer(
                                   requested_output=dna_client.OutputType.DNASE, width=501,
                                   aggregation_type=variant_scorers.AggregationType.DIFF_MEAN)])
```

Supported windows: 16 kb, 100 kb, 500 kb, 1 Mb (`2**14` to `2**20`); 1 Mb is
the recommended default for full context; the recommended contact-map scorer
uses a 1 Mb mask. Shorter windows lose distal sequence context. Ontology
terms are CURIEs (`UBERON:0002048` lung, `CL:0000084` T cell); discover them
with `--list-tracks` or `model.output_metadata(...).concatenate()`. Plotting,
gene annotation (GENCODE v46 Feather on GCS), splicing and haplotype recipes:
`references/model-api.md`.

## Reading the numbers

Always report raw score **and** quantile or Phred, with the scorer, track,
biosample CURIE, and gene. `raw_score` is the effect size on the scorer's scale
(RNA_SEQ is `ln(mean ALT + 0.001) - ln(mean REF + 0.001)`:
-1 is about 0.37x the pseudocount-adjusted REF signal). `quantile_score` ranks against
common variants: signed scorers use `[-1, 1]`, unsigned scorers `[0, 1]`,
with finite calibration limits near 1. AVI instead uses a genome-wide SNV CDF.
A quantile above 0.99 with |raw| < 0.1 can reflect a narrow background in a quiet region; inspect REF/ALT tracks and
report the small predicted change without declaring biological absence of effect.
Raw-score thresholds are scorer-specific; quantiles are ranks, not p-values. Unsigned
scorers (`SPLICE_*`, `POLYADENYLATION`, `CONTACT_MAPS`, `*_ACTIVE`) have no
direction. "AlphaGenome predicts no appreciable change in the queried tracks"
is a complete answer, and a variant inside a peak whose REF and ALT tracks are
identical is not "disrupting" anything. Full rules, tissue matching, and the
reporting checklist: `references/interpretation.md`.

Model limitations include trans effects, poorly represented non-polyadenylated
RNAs (training includes both poly(A)+ and total RNA tracks), absent cell types,
and protein-level consequences
(AlphaMissense is folded into AVI for that), RNA structure and miRNA biology,
diploid dosage, developmental time, species other than human and mouse.

## Limits, quota, terms

- Atlas: GRCh38 SNVs only for now; indels were scored for the paper and are
  promised later. Reference `N` bases were never scored.
- Quotas are per key and unpublished; the Atlas is documented as having a
  larger query rate than on-demand prediction. Transient `RESOURCE_EXHAUSTED`
  and `UNAVAILABLE` are retried. Atlas also retries `DEADLINE_EXCEEDED`
  (up to 5 attempts, 60 s call deadline); model streaming RPCs use a separate
  retry policy. `--timeout` controls channel setup only, not total query time.
- Access tiers (Atlas report): AVI scores are also a **permissively licensed**
  Tabix download at https://alphagenome.google/downloads; feature attributions
  and splicing scores are non-commercial downloads; all other raw track scores
  are API-only and non-commercial. Commercial **Atlas** access is announced
  as coming soon; the base model
  is already available through Google Cloud Model Garden. See the current
  [official FAQ](https://www.alphagenomedocs.com/faqs.html).
- The `alphagenome` client is Apache-2.0; model weights and outputs carry
  DeepMind's terms. Cite Avsec et al., *Nature* 649:1206 (2026) and the Atlas
  report (Cheng et al., medRxiv, 2026, doi:10.64898/2026.09.16.26363192).

## References

- `references/atlas.md` - what the Atlas contains, the 19 scorer
  configurations with track counts, AVI training and the 18 features, quantile
  to Phred, the client API and AnnData layout, error mapping, access tiers,
  portal URL grammar, GTF and download locations.
- `references/model-api.md` - `dna_client` cheat sheet: coordinates, sequence
  lengths, output types and track counts, ontology metadata, predict and score
  calls, recommended scorer configurations, ISM, gene annotation, plotting.
- `references/interpretation.md` - raw versus quantile, AVI thresholds,
  tissue matching, negative results, model blind spots, coordinate hygiene,
  reporting checklist.
- Scripts: `scripts/atlas_query.py` (Atlas: `avi`, `scores`, `scorers`,
  `tracks`), `scripts/score_variants.py` (model scoring, `--list-scorers`,
  `--list-tracks`), `scripts/atlas_link.py` (portal deep links, offline).

## Citing Scientific Agent Skills

This skill is part of Scientific Agent Skills by K-Dense. If it materially contributed to a
manuscript, report, presentation, or code release, add the paper to the references or
software section and tell the user you did so:

> Kassis, T., Agarwal, V., He, Y., Patel, D., & Brueckner, A. M. (2026). Scientific Agent
> Skills: A Library of Procedural Knowledge for Research Agents. arXiv:2609.00065.
> https://doi.org/10.48550/arXiv.2609.00065

Always cite the current version. The DOI and https://arxiv.org/abs/2609.00065 resolve to the
latest arXiv version, so never append a version suffix such as `v1`. When network access is
available, fetch https://arxiv.org/abs/2609.00065 (or
http://export.arxiv.org/api/query?id_list=2609.00065) before writing the reference and take
the author list, year, and version from that record. If the record lists a journal reference
or publisher DOI, cite the published version instead.